Super resolution microscopy of living cells
We use live-cell super-resolution microscopy to visualize subcellular structures and organelle dynamics beyond the diffraction limit. By integrating advanced imaging techniques with biochemical and molecular approaches, we investigate the mechanisms that govern organelle communication and metabolic coordination.
Through this interdisciplinary
approach, we aim to address fundamental questions in cell biology: How does
organelle architecture emerge? What regulates ER鈥搈itochondria interactions? And
how do these processes shape lipid metabolism and support cellular adaptation?
For more information, visit our
website
The image shows a section of a living human cancer cell in which the endoplasmic reticulum has been fluorescently labeled. The cell was imaged using Stimulated Emission Depletion (STED) super-resolution microscopy. The optical resolution enhancement reveals fine structural details that would remain hidden using conventional confocal laser scanning microscopy.
2024
Elancheliyan, P., Maruszczak, K. K., Serwa, R.A., Stephan,
T., Gulgec, A. S., Borrero-Landazabal, M. A., Ngati S., Gosk, A., Jakobs,
S., Wasilewski, M., Chacinska, A: "OCIAD1 and prohibitins regulate the
stability of the TIM23 protein translocase".
Cell Reports 2024
Dec 24; 43(12):115038 (Article).
2024
Scholz, J., Stephan,
T., P茅rez, A. G., Csisz谩r, A., Hersch, N., Fischer, L. S., Br眉hmann, S.,
K枚rber, S., Litschko, C., Mijanovic, L., Kaufmann, T., Lange, F., Springer, R.,
Pich, A., Jakobs, S., Peckham, M., Tarantola, M., Grashoff, C., Merkel, R.,
Faix, J.: "Decisive role of mDia-family formins in cell cortex function
of highly adherent cells".
Science
Advances 2024 Nov;10(44):eadp5929 (Article).
2024
Stephan, T., Ilgen, P., Jakobs, S.: "Visualizing
mitochondrial dynamics at the nanoscale"
Light Science & Applications (News and Views).
2024
Chen, J., Stephan,
T., Gaedke, F., Liu, T., Li, Y., Schauss, A., Chen, P., Wulff, V., Jakobs,
S., J眉ngst, C., Chen, Z.: "An aldehyde-crosslinking mitochondrial probe
for STED imaging in fixed cells".
Proceedings of
the National Academy of Sciences 121,
e2317703121 (Article).
2024
Stephan, T.*, Stoldt, S.*,
Barbot, M.*, Carney, T.D., Lange, F., Bates, M., Bou Dib, P., Inamdar, K.,
Shcherbata, H.R., Meinecke, M., Riedel, D., Dennerlein, S., Rehling, P.,
Jakobs, S.: "Drosophila MIC10b can polymerize into cristae-shaping
filaments"'.
Life Science
Alliance 7, e202302177 (Article).
2023
Ebrahimi, V., Stephan,
T., Kim, J., Carravilla, P., Eggeling, C., Jakobs, S., Han, K.Y.: "Deep
learning enables fast, gentle STED microscopy".
Communications Biology 6, 674
(Article).
2022
Liu, T.*, Stephan,
T.*, Chen, P., Keller-Findeisen, J., Chen, J., Riedel, D., Yang, Z.,
Jakobs, S., Chen, Z.: "Multi-color live-cell STED nanoscopy of
mitochondria with a gentle inner membrane stain".
Proceedings of
the National Academy of Sciences 119,
e2215799119 (Article).
2022
Bates, M.,
Keller-Findeisen, J., Przybylski, A., H眉per, A., Stephan, T., Ilgen, P.,
Cereceda Delgado, A.R., D'Este, E., Egner, A., Jakobs, S., Sahl, S.J., Hell,
S.W.: "Optimal precision and accuracy in 4Pi-STORM using dynamic spline
PSF models".
Nature Methods 19, 603鈥�612
(Article).
2020
Stephan, T.*, Br眉ser, C.*,
Deckers, M., Steyer, A.M., Balzarotti, F., Barbot, M., Behr, T.S., Heim, G.,
H眉bner, W., Ilgen, P., Lange, F., Pacheu-Grau, D., Pape, J.K., Stoldt, S.,
Huser, T., Hell, S.W., M枚bius, W., Rehling, P., Riedel, D., Jakobs, S.: "MICOS
assembly controls mitochondrial inner membrane remodeling and crista junction
redistribution to mediate cristae formation".
The EMBO
Journal 39, e104105 (Article).
2020
Pape, J.K.*, Stephan, T.*,
Balzarotti, F., B眉chner, R., Lange, F., Riedel, D., Jakobs, S., Hell, S.W.:
"Multicolor 3D MINFLUX nanoscopy of mitochondrial MICOS proteins". Proceedings of the National
Academy of Sciences 117, 20607鈥�20614 (Article).
2020
Jakobs, S., Stephan,
T., Ilgen, P., Br眉ser, C.: "Light Microscopy of Mitochondria at the
Nanoscale".
Annual Review
of Biophysics (Review).
2019
Stephan, T., R枚sch, A.,
Riedel, D., Jakobs, S.: "Live-cell STED nanoscopy of mitochondrial
cristae".
Scientific
Reports 9, 12419 (Article).
2019
Stoldt, S.*, Stephan,
T.*, Jans, D.C., Br眉ser, C., Lange, F., Keller-Findeisen, J., Riedel, D.,
Hell, S.W., Jakobs, S.: "Mic60 exhibits a coordinated clustered
distribution along and across yeast and mammalian mitochondria".
Proceedings of
the National Academy of Sciences 116, 9853
(Article).
*) These authors contributed equally.